6U0I
Putative Antitoxin HicB3 from Escherichia coli str. K-12 substr. DH10B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-14 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.045, 86.476, 122.888 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 49.730 - 1.900 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.789 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.730 | 49.730 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.127 | 0.107 | 0.548 |
| Rmeas | 0.138 | 0.117 | 0.651 |
| Rpim | 0.053 | 0.045 | 0.346 |
| Number of reflections | 48305 | 2573 | 1847 |
| <I/σ(I)> | 5.5 | 1.69 | |
| Completeness [%] | 96.6 | 100 | 75 |
| Redundancy | 6 | 6.7 | 3 |
| CC(1/2) | 0.992 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M sodium chloride, 25% PEG 3350, 0.1 M Bis-Tris:HCl |
