6U0A
Human Angiopoietin-Like 4 C-Terminal Domain (cANGPTL4) with Glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.977 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 133.489, 134.868, 39.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.160 - 2.110 |
R-factor | 0.2159 |
Rwork | 0.214 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z3s |
RMSD bond length | 0.006 |
RMSD bond angle | 1.031 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.440 | 2.150 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.083 | 0.639 |
Rmeas | 0.091 | 0.700 |
Rpim | 0.036 | 0.280 |
Number of reflections | 20254 | 922 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 98.3 | 99.2 |
Redundancy | 6.3 | 6 |
CC(1/2) | 0.995 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 0.1 M HEPES 7.5, 0.1 M sodium chloride, and 1.6 M ammonium sulfate This solution with 20% glycerol was used as a cryoprotectant |