6U0A

Human Angiopoietin-Like 4 C-Terminal Domain (cANGPTL4) with Glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.977
Spacegroup name	C 2 2 21
Unit cell lengths	133.489, 134.868, 39.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.160 - 2.110
R-factor	0.2159
Rwork	0.214
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z3s
RMSD bond length	0.006
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.150
High resolution limit [Å]	2.110	2.110
Rmerge	0.083	0.639
Rmeas	0.091	0.700
Rpim	0.036	0.280
Number of reflections	20254	922
<I/σ(I)>	13.5
Completeness [%]	98.3	99.2
Redundancy	6.3	6
CC(1/2)	0.995	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.1 M HEPES 7.5, 0.1 M sodium chloride, and 1.6 M ammonium sulfate This solution with 20% glycerol was used as a cryoprotectant