6U05
Crystal Structure of Fungal RNA Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.747, 44.690, 79.500 |
Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
Resolution | 39.675 - 1.948 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u32 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.948 | 5.290 | 1.950 |
Rmerge | 0.068 | 0.032 | 0.556 |
Rmeas | 0.078 | 0.037 | 0.663 |
Rpim | 0.037 | 0.017 | 0.356 |
Number of reflections | 38436 | 2052 | 1652 |
<I/σ(I)> | 7.6 | ||
Completeness [%] | 96.3 | 97.3 | 83.1 |
Redundancy | 3.9 | 4.1 | 2.7 |
CC(1/2) | 0.999 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M KH2PO4, 20% PEG3350 |