6U05
Crystal Structure of Fungal RNA Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.747, 44.690, 79.500 |
| Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
| Resolution | 39.675 - 1.948 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u32 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.948 | 5.290 | 1.950 |
| Rmerge | 0.068 | 0.032 | 0.556 |
| Rmeas | 0.078 | 0.037 | 0.663 |
| Rpim | 0.037 | 0.017 | 0.356 |
| Number of reflections | 38436 | 2052 | 1652 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 96.3 | 97.3 | 83.1 |
| Redundancy | 3.9 | 4.1 | 2.7 |
| CC(1/2) | 0.999 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M KH2PO4, 20% PEG3350 |
