6U04
Crystal structure of human BRPF1 PZP bound to histone H3 tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-30 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 72.461, 72.461, 155.297 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.684 - 2.200 |
R-factor | 0.1756 |
Rwork | 0.172 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5erc |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.196 | 4.740 | 2.200 |
Rmerge | 0.144 | 0.066 | 0.794 |
Rmeas | 0.148 | 0.067 | 0.814 |
Rpim | 0.032 | 0.014 | 0.177 |
Number of reflections | 12975 | 1446 | 1249 |
<I/σ(I)> | 6 | ||
Completeness [%] | 99.5 | 98.1 | 100 |
Redundancy | 20.8 | 21.4 | 20.7 |
CC(1/2) | 0.999 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.2 M Lithium sulfate, 0.1 M Tris-HCl pH 8.5, 40% (v/v) PEG400, and 0.01M Praseodymium(III) acetate hydrate |