6U00

Crystal Structure of Fungal RNA Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	55.436, 122.873, 150.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.907 - 1.981
R-factor	0.1951
Rwork	0.193
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u32
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.055	0.029	0.618
Rmeas	0.060	0.032	0.676
Rpim	0.024	0.013	0.269
Total number of observations	211528
Number of reflections	34731	1951	1281
<I/σ(I)>	10.5
Completeness [%]	95.8	99.1	73.7
Redundancy	6.1	5.8	5.4
CC(1/2)		0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.3M NaH2PO4, 15%PEG3350