6TZW

Coiled-coil registry shifts in the F684I mutant of Bicaudal D result in cargo-independent activation of dynein motility

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	60.045, 60.045, 142.637
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.450 - 2.350
R-factor	0.2514
Rwork	0.251
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bl6
RMSD bond length	0.003
RMSD bond angle	0.454
Data reduction software	XDS (Implemented in RAPD, a software suite developed by APS)
Data scaling software	Aimless (Implemented in RAPD, a software suite developed by APS)
Phasing software	PHENIX (1.16-3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.650	2.434
High resolution limit [Å]	2.350	2.350
Rmerge	0.044	1.621
Rmeas	0.047
Rpim	0.015	0.549
Number of reflections	12955	1263
<I/σ(I)>	28	1.26
Completeness [%]	99.4	99.92
Redundancy	9.7	9.6
CC(1/2)	1.000	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	4% PEG 3350, 0.4 M NaSCN, 5% glycerol