6TZC

Crystal Structure of African Swine Fever Virus A179L with the Autophagy Regulator Beclin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	54.565, 44.347, 129.022
Unit cell angles	90.00, 94.53, 90.00

Refinement procedure

Resolution	51.580 - 2.410
R-factor	0.2159
Rwork	0.214
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ua5
RMSD bond length	0.005
RMSD bond angle	0.590
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	AMBER (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.590	2.470
High resolution limit [Å]	2.410	2.410
Rmerge	0.120	0.520
Number of reflections	24021	2373
<I/σ(I)>	4.6	1.4
Completeness [%]	99.4	99.7
Redundancy	2.9	2.7
CC(1/2)	0.980	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2M Ammonium sulfate, 0.1M Bis-Tris pH 5.5, 25% PEG 3350