6TZA
CryoEM reconstruction of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 7.2 |
| Software | RELION,SerialEM,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
