6TZ3

Crystal Structure of Human Synaptotagmin 1 C2B without Ca2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.097
Spacegroup name	P 32 2 1
Unit cell lengths	54.594, 54.594, 103.904
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.400 - 1.170
R-factor	0.1561
Rwork	0.155
R-free	0.17500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uow
RMSD bond length	0.013
RMSD bond angle	1.245
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.190
High resolution limit [Å]	1.170	3.180	1.170
Rmerge	0.045	0.021	0.299
Rmeas	0.045	0.024	0.337
Rpim	0.020	0.011	0.151
Number of reflections	59591	2199	3010
<I/σ(I)>	16.4
Completeness [%]	97.2	66.4	99.4
Redundancy	4.7	4.3	4.6
CC(1/2)	0.999	0.999	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.2M Ammonium Sulfate, 30% PEG3350