6TYZ

Structure of Ku80 von Willebrand domain complexed with APLF Ku Binding Motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-01
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	43.807, 71.189, 79.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.443 - 1.511
R-factor	0.179194856937
Rwork	0.178
R-free	0.20256
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tyv
RMSD bond length	0.007
RMSD bond angle	0.832
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.540
High resolution limit [Å]	1.510	1.510
Rmeas	0.049	0.540
Number of reflections	39709	1922
<I/σ(I)>	50
Completeness [%]	99.8
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M HEPES pH 7.5, 20% (w/v) PEG4000