6TYV
Structure of Ku80 von Willebrand domain complexed with WRN Ku Binding Motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-01 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.112, 71.351, 79.606 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.520 - 1.926 |
R-factor | 0.194656196721 |
Rwork | 0.193 |
R-free | 0.23484 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jeq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.865 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmeas | 0.070 | 0.496 |
Number of reflections | 18919 | 914 |
<I/σ(I)> | 34.3 | |
Completeness [%] | 99.5 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M Na-HEPES pH 7.5, 25 % (w/v) PEG3000 |