6TYU
Structure of Ku80 von Willebrand domain complexed with MRI Ku Binding Motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-01 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.574, 71.350, 79.163 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.803 - 1.469 |
R-factor | 0.179161282252 |
Rwork | 0.178 |
R-free | 0.20077 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6tyv |
RMSD bond length | 0.008 |
RMSD bond angle | 0.905 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.468 | 1.470 |
Rmeas | 0.048 | 0.602 |
Number of reflections | 43410 | 1900 |
<I/σ(I)> | 53 | |
Completeness [%] | 99.1 | |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Potassium formate, 20 % (w/v) PEG3350 |