6TYT
Structure of Ku80 von Willebrand domain S229A mutant complexed with APLF and XLF Ku Binding Motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-07 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 43.642, 79.407, 146.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.888 - 2.403 |
R-factor | 0.226705772622 |
Rwork | 0.225 |
R-free | 0.25953 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6tyz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.378 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmeas | 0.410 | 0.643 |
Number of reflections | 10611 | 410 |
<I/σ(I)> | 43 | |
Completeness [%] | 97.0 | |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M HEPES NaOH pH 7.5, 25 % (w/v) PEG3350 |