6TYT
Structure of Ku80 von Willebrand domain S229A mutant complexed with APLF and XLF Ku Binding Motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-07 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.642, 79.407, 146.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.888 - 2.403 |
| R-factor | 0.226705772622 |
| Rwork | 0.225 |
| R-free | 0.25953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tyz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmeas | 0.410 | 0.643 |
| Number of reflections | 10611 | 410 |
| <I/σ(I)> | 43 | |
| Completeness [%] | 97.0 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M HEPES NaOH pH 7.5, 25 % (w/v) PEG3350 |
