6TY0

NT PART CRYSTAL STRUCTURE OF THE RYMV-ENCODED VIRAL RNA SILENCING SUPPRESSOR P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	F 41 3 2
Unit cell lengths	195.612, 195.612, 195.612
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	112.940 - 2.100
R-factor	0.18564
Rwork	0.184
R-free	0.21417
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.709
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.940	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.055	0.880
Rmeas	0.058
Rpim	0.012
Number of reflections	22243	3160
<I/σ(I)>	45	6
Completeness [%]	100.0	100
Redundancy	20
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		293	20 mM Tris pH 8, 75 mM NaCl, 1 mM DTT and 300 mM ZnCl2