6TXX
CRYSTAL STRUCTURE OF HUMAN FKBP51 FK1 DOMAIN A19T MUTANT IN COMPLEX WITH SAFit2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000030 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 48.294, 48.294, 186.883 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.170 - 2.100 |
R-factor | 0.1924 |
Rwork | 0.190 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o5q |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.290 | 2.107 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.148 | 1.044 |
Rmeas | 0.156 | 1.106 |
Rpim | 0.051 | 0.362 |
Number of reflections | 15619 | 120 |
<I/σ(I)> | 12.8 | 2.1 |
Completeness [%] | 100.0 | 97.6 |
Redundancy | 9.2 | 9.4 |
CC(1/2) | 0.998 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 28-43% PEG3350, 0.2M NH4OAc, 0.1M HEPES |