6TXN

Crystal structure of thermotoga maritima Ferritin in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978631
Spacegroup name	H 3 2
Unit cell lengths	176.020, 176.020, 356.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.276 - 2.010
R-factor	0.1935
Rwork	0.193
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	7.780
High resolution limit [Å]	2.010	2.010
Rmerge	0.084	1.526
Number of reflections	140330	13704
<I/σ(I)>	11
Completeness [%]	99.9	100
Redundancy	6.5
CC(1/2)	0.996	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 M MgCl2, 2.4 M (NH4)2 SO4, 0.1M MES. pH 6.0. 1ul of 10mg/mL protein was mixed with same amount of well solution, crystals normally appears in 2 days