6TXI

Crystal structure of thermotoga maritima E65A Ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	H 3 2
Unit cell lengths	175.700, 175.700, 353.568
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.938 - 1.759
R-factor	0.1803
Rwork	0.180
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.15.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.938	48.938	1.870
High resolution limit [Å]	1.759	5.250	1.760
Rmerge	0.085	0.022	2.403
Rmeas	0.089	0.023	2.532
Number of reflections	206460	8111	33052
<I/σ(I)>	19.79	84.55	0.94
Completeness [%]	99.9	99.8	99.6
Redundancy	10.187	9.889	10.122
CC(1/2)	1.000	1.000	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2.4 M (NH4)2 SO4, 0.1M MES. pH 6.0.