6TXH

Crystal structure of thermotoga maritima Ferritin in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.9184
Spacegroup name	H 3 2
Unit cell lengths	176.172, 176.172, 351.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.068 - 2.198
R-factor	0.205
Rwork	0.204
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.068	25.068	2.330
High resolution limit [Å]	2.198	6.540	2.200
Rmerge	0.121	0.031	3.391
Rmeas	0.128	0.032	3.568
Number of reflections	106131	4246	16843
<I/σ(I)>	13.58	62.12	0.63
Completeness [%]	99.6	99.5	98.6
Redundancy	10.189	10.533	10.35
CC(1/2)	0.999	1.000	0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2 M MgCl2, 2.4 M (NH4)2 SO4, 0.1M MES. pH 6.0. 1ul of 10mg/mL protein was mixed with same amount of well solution, crystals normally appears in 2 days