6TWY

MAGI1_2 complexed with a phosphomimetic RSK1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	61.450, 96.380, 198.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.190 - 2.300
R-factor	0.2068
Rwork	0.205
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d
RMSD bond length	0.004
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	52853	3836
<I/σ(I)>	19.97	1.18
Completeness [%]	99.0
Redundancy	13.5
CC(1/2)	1.000	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	22% PEG3000, 100mM Na-citrate (pH 5.5), 100mM Na-citrate