6TWX
MAGI1_2 complexed with a phosphorylated 16E6 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 61.470, 96.550, 198.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.910 - 2.300 |
R-factor | 0.2116 |
Rwork | 0.209 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n7d |
RMSD bond length | 0.004 |
RMSD bond angle | 0.672 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 52873 | 3833 |
<I/σ(I)> | 15.33 | |
Completeness [%] | 98.8 | |
Redundancy | 27.1 | |
CC(1/2) | 1.000 | 0.561 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 22% PEG3000, 100mM Na-citrate (5.5), 100mM Na-citrate |