6TWQ

MAGI1_2 complexed with a 16E6 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	60.810, 97.130, 201.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.560 - 2.650
R-factor	0.2399
Rwork	0.239
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d
RMSD bond length	0.003
RMSD bond angle	0.536
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.580	2.720
High resolution limit [Å]	2.650	2.650
Number of reflections	35247	2554
<I/σ(I)>	15.45	1.39
Completeness [%]	99.2
Redundancy	13.5
CC(1/2)	0.999	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	22% PEG3000, 100 mM Na-citrate (pH 5.5), 100 mM Na-citrate