6TVY
Structure of hen egg white lysozyme crystallized in the presence of Tb-Xo4 crystallophore in the XtalController device
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-07-03 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.810, 78.810, 38.332 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.240 - 1.510 |
R-factor | 0.145 |
Rwork | 0.141 |
R-free | 0.17490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f2i |
RMSD bond length | 0.004 |
RMSD bond angle | 0.712 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.240 | 1.600 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmeas | 0.036 | 0.726 |
Number of reflections | 36571 | 5936 |
<I/σ(I)> | 29.7 | 1.9 |
Completeness [%] | 99.2 | 96.5 |
Redundancy | 8.4 | 4.8 |
CC(1/2) | 1.000 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | Lysozyme stock solution at 50 mg/ml in 10 mM Sodium Acetate pH 4.5, 40 mM NaCl, incubated in the XtalController instrument. The crystallization was triggered by the addition of 10mM Tb-Xo4 crystallophore. |