6TVY

Structure of hen egg white lysozyme crystallized in the presence of Tb-Xo4 crystallophore in the XtalController device

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-07-03
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.810, 78.810, 38.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.240 - 1.510
R-factor	0.145
Rwork	0.141
R-free	0.17490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f2i
RMSD bond length	0.004
RMSD bond angle	0.712
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.240	1.600
High resolution limit [Å]	1.510	1.510
Rmeas	0.036	0.726
Number of reflections	36571	5936
<I/σ(I)>	29.7	1.9
Completeness [%]	99.2	96.5
Redundancy	8.4	4.8
CC(1/2)	1.000	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	Lysozyme stock solution at 50 mg/ml in 10 mM Sodium Acetate pH 4.5, 40 mM NaCl, incubated in the XtalController instrument. The crystallization was triggered by the addition of 10mM Tb-Xo4 crystallophore.