6TVT
Crystal structure of the haemagglutinin mutant (Gln226Leu, Del228) from an H10N7 seal influenza virus isolated in Germany in complex with human receptor analogue 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.014, 211.830, 82.224 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 79.530 - 2.200 |
| R-factor | 0.2363 |
| Rwork | 0.235 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.250 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 116210 | 5758 |
| <I/σ(I)> | 13.8 | 1.9 |
| Completeness [%] | 98.6 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.994 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 10% PEG6000, 0.1M bicine pH9.0 |
