6TVP

Structure of Mycobacterium smegmatis alpha-maltose-1-phosphate synthase GlgM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	135.371, 144.928, 46.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.460 - 1.900
R-factor	0.1751
Rwork	0.173
R-free	0.21170
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.581
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.460	3.590	1.940
High resolution limit [Å]	1.900	3.500	1.900
Rmerge	0.088	1.459	2.102
Rmeas	0.092	1.474	2.187
Number of reflections	73208	810	4488
<I/σ(I)>	18.8	3.9	1.4
Completeness [%]	96.3	99.9	95.8
Redundancy	13.3		13.2
CC(1/2)	1.000	0.870	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% (w/v) PEG 3350, 0.2 M malonate and 100 mM Bis-Tris Propane, pH 6.5
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% (w/v) PEG 3350, 0.2 M malonate and 100 mM Bis-Tris Propane, pH 6.5