6TVL

Hen Egg White Lysozyme in complex with a "half sandwich"-type Ru(II) coordination compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	79.270, 79.270, 35.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.451 - 1.395
R-factor	0.2221
Rwork	0.221
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.451	1.445
High resolution limit [Å]	1.395	1.395
Rmerge	0.021	0.181
Rmeas	0.029	0.256
Rpim	0.021	0.181
Number of reflections	23013	2192
<I/σ(I)>	15	3.75
Completeness [%]	98.5	95.35
Redundancy	2	2
CC(1/2)	0.999	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	NaCl 0.7-1.5 M, 0.1 M Na acetate