6TVK

Alpha-L-fucosidase isoenzyme 2 from Paenibacillus thiaminolyticus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 64 2 2
Unit cell lengths	125.118, 125.118, 231.905
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.080 - 2.100
Rwork	0.189
R-free	0.22520
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.553
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.141	3.570
Rmeas	0.144	3.653
Rpim	0.031	0.770
Number of reflections	63022	4375
<I/σ(I)>	25.5	1.9
Completeness [%]	99.7	100
Redundancy	40.1	42.3
CC(1/2)	1.000	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	283.15	Condition: 0.1 M NaCl, 1.5 M ammonium acetate, 0.1 M BIS-TRIS pH 6.5 The condition was diluted to 80% 5 mM maltose as an additive