6TVC
Crystal structure of the haemagglutinin from a transmissible H10N7 seal influenza virus isolated in Netherland
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.380, 214.950, 74.670 |
| Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
| Resolution | 51.480 - 1.840 |
| R-factor | 0.233 |
| Rwork | 0.232 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.480 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 171611 | 8592 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 26%-31% PEG600, 0.1M HEPES pH7.5 or 0.1M tris pH8.0 |
