6TV9
Heme d1 biosynthesis associated Protein NirF in complex with dihydro-heme d1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.7265 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.197, 149.443, 110.174 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 100.090 - 1.893 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.25760 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6tv2 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev 3742) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.090 | 2.140 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rpim | 0.076 | 0.342 |
| Number of reflections | 149789 | 7472 |
| <I/σ(I)> | 6.1 | 1.9 |
| Completeness [%] | 93.0 | 52.6 |
| Redundancy | 6.1 | 3.3 |
| CC(1/2) | 0.991 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% (w/v) PEG 4000 20% (v/v) glycerol 0.02M Morpheus amino acid Mix 0.1M MOPS/HEPES pH 7.5 |
