6TV7

Crystal structure of rsGCaMP in the OFF state (illuminated)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999995
Spacegroup name	P 41 21 2
Unit cell lengths	120.670, 120.670, 96.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.090 - 2.900
R-factor	0.1855
Rwork	0.182
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ek4
RMSD bond length	0.010
RMSD bond angle	1.897
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	16254	1174
<I/σ(I)>	11.17	1.6
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.996	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.21 M sodium formate, 0.1 M Bis-Tris-Propane buffer pH 8.5, 18% (w/v) PEG 3350