6TUW
human XPG-DNA, Complex 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 129.510, 129.510, 118.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.790 - 3.500 |
| R-factor | 0.2665 |
| Rwork | 0.265 |
| R-free | 0.29320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tur |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.684 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 3.625 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 6627 | 1536 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 15.9 | |
| CC(1/2) | 0.998 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 5% PEG 3350, 50 mM Na Citrate pH 4.0 |
