6TUV
Crystal structure of Mindy1 in complex with Lys48 linked di-ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.939274 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 73.862, 73.862, 202.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.370 - 2.160 |
R-factor | 0.2096 |
Rwork | 0.207 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jkn 1ubq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.608 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.810 | 2.230 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.087 | 0.955 |
Rmeas | 0.094 | 1.028 |
Rpim | 0.036 | 0.378 |
Total number of observations | 212300 | |
Number of reflections | 31102 | 2629 |
<I/σ(I)> | 13.9 | 4.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.8 | 7.3 |
CC(1/2) | 0.999 | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 100 mM Tris-HCl, 200mM LiSO4.H2O, 30% PEG400 |