6TUK
Crystal structure of Fdr9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 142.791, 142.791, 142.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.480 - 1.900 |
| R-factor | 0.1848 |
| Rwork | 0.184 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1w |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (dev-370) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 142.790 | 142.790 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.158 | 0.035 | 2.742 |
| Rmeas | 0.159 | 0.035 | 2.766 |
| Rpim | 0.018 | 0.004 | 0.363 |
| Total number of observations | 2946416 | 28051 | 144375 |
| Number of reflections | 39768 | 464 | 2527 |
| <I/σ(I)> | 25 | 97.4 | 2 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 74.1 | 60.5 | 57.1 |
| CC(1/2) | 1.000 | 1.000 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M NaCl 0.1M Bis-Tris pH6.5 1.5M (NH4)2SO4 |
