6TUK

Crystal structure of Fdr9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 41 3 2
Unit cell lengths	142.791, 142.791, 142.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.480 - 1.900
R-factor	0.1848
Rwork	0.184
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q1w
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	MrBUMP
Refinement software	PHENIX (dev-370)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	142.790	142.790	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.158	0.035	2.742
Rmeas	0.159	0.035	2.766
Rpim	0.018	0.004	0.363
Total number of observations	2946416	28051	144375
Number of reflections	39768	464	2527
<I/σ(I)>	25	97.4	2
Completeness [%]	100.0	100	100
Redundancy	74.1	60.5	57.1
CC(1/2)	1.000	1.000	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M NaCl 0.1M Bis-Tris pH6.5 1.5M (NH4)2SO4