6TUA

The RYK Pseudokinase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	PSI PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	I 1 2 1
Unit cell lengths	60.645, 47.242, 152.741
Unit cell angles	90.00, 97.64, 90.00

Refinement procedure

Resolution	37.850 - 2.380
R-factor	0.1966
Rwork	0.194
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tc0
RMSD bond length	0.008
RMSD bond angle	0.879
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.850	2.470
High resolution limit [Å]	2.380	2.380
Number of reflections	17214	1718
<I/σ(I)>	8.3	2
Completeness [%]	98.6	97
Redundancy	3.7
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M lithium sulfate, 0.1 M HEPES 7.5