6TU2

Crystal structure of rat annexin A11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	39.070, 86.690, 87.300
Unit cell angles	114.00, 101.98, 97.21

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.23
Rwork	0.228
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ann
RMSD bond length	0.002
RMSD bond angle	0.430
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.300	2.300
Rmeas	0.209	1.966
Number of reflections	42245	3046
<I/σ(I)>	5.2	1.1
Completeness [%]	95.4	95
Redundancy	3.3	3.2
CC(1/2)	0.992	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	100 mM Bis-tris pH 5.5, 3 M NaCl