6TTJ
Neutral invertase 2 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.450, 186.708, 152.200 |
| Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
| Resolution | 46.654 - 3.392 |
| R-factor | 0.2537 |
| Rwork | 0.252 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5goo |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.460 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.415 | 1.698 |
| Number of reflections | 79402 | 4000 |
| <I/σ(I)> | 4.8 | 1.2 |
| Completeness [%] | 99.0 | 87.6 |
| Redundancy | 3.7 | 3.3 |
| CC(1/2) | 0.950 | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 298 | 2.9 M NaCl, 9% polyethylene glycol 4000, 0.1 M bicine, pH 9.0 |
