6TSC
OphMA I407P complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.379, 89.379, 157.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.330 - 2.190 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n0o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.518 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.330 | 2.230 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmeas | 0.058 | 2.088 |
| Number of reflections | 38116 | 1877 |
| <I/σ(I)> | 15.7 | 1.1 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 11.1 | 10.9 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.3-0.4 M KSCN, 0.1 M Bicine pH 8-9, 1.8-2.5 M sodium malonate |
