6TS3

EF-hands 3 and 4 of alpha-actinin in complex with CaMKII regulatory segment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	43.355, 47.270, 47.472
Unit cell angles	90.00, 110.53, 90.00

Refinement procedure

Resolution	44.460 - 1.280
R-factor	0.1839
Rwork	0.183
R-free	0.19890
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.698
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.460	1.300
High resolution limit [Å]	1.280	1.280
Rmerge	0.035	0.437
Rmeas	0.038	0.487
Number of reflections	46303	2152
<I/σ(I)>	19.7	2.4
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.999	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350