6TRZ
Crystal structure of horse L ferritin (HoLf) Fe(III)-loaded for 15 minutes
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.471, 180.471, 180.471 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.888 - 2.020 |
| Rwork | 0.165 |
| R-free | 0.18780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lg2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.230 | 2.130 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.090 | 0.877 |
| Rmeas | 0.094 | 0.914 |
| Rpim | 0.020 | 0.194 |
| Number of reflections | 17109 | 2448 |
| <I/σ(I)> | 19.8 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.6 | 22 |
| CC(1/2) | 0.999 | 0.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 30 mM cadmium nitrate, 1 M ammonium sulfate, and 200 mM sodium acetate, pH 5.5 |
