6TRU
Crystal structure of the N-terminal half of the TFIIH subunit p52
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 31 |
| Unit cell lengths | 104.309, 104.309, 164.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.970 - 2.800 |
| R-factor | 0.1875 |
| Rwork | 0.186 |
| R-free | 0.20850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.438 |
| Data reduction software | Aimless |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.970 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.162 | 2.829 |
| Rpim | 0.053 | 0.901 |
| Number of reflections | 49518 | 4628 |
| <I/σ(I)> | 10.7 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10.8 |
| CC(1/2) | 0.997 | 0.325 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293.15 | calcium acetate, PEG 8000, HEPES |
