6TRS

Crystal structure of TFIIH subunit p52 in complex with p8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	74.216, 85.954, 92.172
Unit cell angles	90.00, 94.93, 90.00

Refinement procedure

Resolution	19.920 - 2.680
R-factor	0.217
Rwork	0.216
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.950
High resolution limit [Å]	2.680	2.680
Rmerge	0.280	1.429
Rpim	0.140	0.715
Number of reflections	17307	865
<I/σ(I)>	5.7	1.2
Completeness [%]	88.7	49.5
Redundancy	4.9	4.8
CC(1/2)	0.983	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293.15	ammonium chloride, PEG 4000, TRIS