6TRN
Crystal structure of HLA A2*01-AVYDGREHTV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.970, 80.690, 58.660 |
| Unit cell angles | 90.00, 115.13, 90.00 |
Refinement procedure
| Resolution | 48.310 - 1.350 |
| R-factor | 0.1627 |
| Rwork | 0.161 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e00 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.467 |
| Data reduction software | xia2 (0.5.328) |
| Data scaling software | xia2 (0.5.328) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.310 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.055 | 1.139 |
| Rpim | 0.028 | 0.659 |
| Total number of observations | 365788 | |
| Number of reflections | 102850 | 5113 |
| <I/σ(I)> | 12.2 | 1 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium sodium tartrate, 0.1 M bis-tris propane pH6.5, 20 % PEG 3350 |
