6TRI

CI-MOR repressor-antirepressor complex of the temperate bacteriophage TP901-1 from Lactococcus lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.972
Spacegroup name	P 3 2 1
Unit cell lengths	94.405, 94.405, 30.558
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.879 - 2.277
R-factor	0.1904
Rwork	0.189
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5A7L + MOR NMR structure (just deposited 6TO6)
RMSD bond length	0.002
RMSD bond angle	0.642
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.700	2.410
High resolution limit [Å]	2.277	2.277
Number of reflections	7285	1184
<I/σ(I)>	7.95
Completeness [%]	99.9
Redundancy	16.1
CC(1/2)	0.996	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M lithium sulfate, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 in reservoir