Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-29 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.80 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.170, 81.636, 91.148 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.885 - 1.000 |
Rwork | 0.101 |
R-free | 0.11610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sil |
RMSD bond length | 0.020 |
RMSD bond angle | 2.381 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | ACORN |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.885 | 1.020 |
High resolution limit [Å] | 1.000 | 1.000 |
Number of reflections | 186230 | 8956 |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 98.3 | 96 |
Redundancy | 5 | 3.5 |
CC(1/2) | 0.999 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Crystals grown by hanging drop vapour diffusion. A 1:1 mixture of 15mg/ml protein solution and an 8:4 mixture of 3.0M K2HPO4 to 1.4M KH2P04 was placed above a well of an 8:6 solution of 3.0M K2HPO4 to 1.4M KH2PO4. Then serially cryoprotected in situ to 40% glycerol (v/v with mother liquor) in 10% increments over a period of a few minutes. |