6TR9
Crystal structure of human L ferritin (HuLf) triple variant E60A-E61A-E64A
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 151.215, 151.215, 151.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.810 - 2.460 |
| Rwork | 0.201 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lg8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.751 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.610 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.106 | 1.044 |
| Rmeas | 0.112 | 1.090 |
| Rpim | 0.024 | 0.233 |
| Number of reflections | 11055 | 1562 |
| <I/σ(I)> | 21.2 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.2 | 21.6 |
| CC(1/2) | 1.000 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 80 mM cadmium nitrate, 80 mM ammonium sulfate, 200 mM sodium acetate, pH 4.6 |
