6TQU

The crystal structure of the MSP domain of human MOSPD2 in complex with the Phospho-FFAT motif of STARD3.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	87.968, 87.968, 95.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.980 - 2.400
R-factor	0.1861
Rwork	0.183
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tqt
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (dev-3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.970	87.970	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.258	0.080	3.976
Rmeas	0.263	0.082	4.054
Rpim	0.053	0.018	0.786
Total number of observations	378156	7355	40984
Number of reflections	15203	364	1559
<I/σ(I)>	9.1	29.9	1
Completeness [%]	100.0	99.9	100
Redundancy	24.9	20.2	26.3
CC(1/2)	0.997	0.997	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	7.5% PEG 2000, 7.5% PEG 3350, 7.5% PEG 4000, 7.5% PEG 5000 MME, 0.05 M Magnesium sulfate, 0.1 M HEPES pH 7.5