6TQP

Structural insight into tanapoxvirus mediated inhibition of apoptosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	54.093, 54.869, 60.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.450 - 1.849
R-factor	0.192246521962
Rwork	0.191
R-free	0.21844
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	previously solved structure of TNP_16L
RMSD bond length	0.006
RMSD bond angle	0.687
Data reduction software	XDS (1.11.1_2575)
Data scaling software	Aimless (7.0.077)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.450	1.890
High resolution limit [Å]	1.849	1.850
Rmerge	0.066	0.072
Number of reflections	15799	15850
<I/σ(I)>	11.1
Completeness [%]	99.5	99.7
Redundancy	5.5	5.5
CC(1/2)	0.990	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Potassium thiocyanate, 30% PEG 2000MME