6TQJ
Crystal structure of the c14 ring of the F1FO ATP synthase from spinach chloroplast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 93.136, 96.336, 158.684 |
| Unit cell angles | 90.00, 106.72, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v3c |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.170 | 48.170 | 2.360 |
| High resolution limit [Å] | 2.300 | 10.030 | 2.300 |
| Rmerge | 0.192 | 0.051 | 1.169 |
| Rmeas | 0.225 | 0.060 | 1.369 |
| Rpim | 0.114 | 0.031 | 0.704 |
| Total number of observations | 214591 | 2592 | 12342 |
| Number of reflections | 57691 | 753 | 3484 |
| <I/σ(I)> | 4.9 | 11.5 | 1.2 |
| Completeness [%] | 96.7 | 98.2 | 75.6 |
| Redundancy | 3.7 | 3.4 | 3.5 |
| CC(1/2) | 0.993 | 0.998 | 0.421 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.1 M MES pH 5.8, 1.4 M ammonium sulfate |
