6TPW

Crystal structures of FNIII domain one through four of the human leucocyte common antigen-related protein ( LAR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	I 41 2 2
Unit cell lengths	74.860, 74.860, 461.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.985 - 2.900
R-factor	0.2821
Rwork	0.281
R-free	0.30120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tpv 6tpu
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.290	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	15285	1489
<I/σ(I)>	12.1
Completeness [%]	98.7
Redundancy	8.6
CC(1/2)	0.999	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	0.2 M Ammonium sulfate and 30% PEG8000