6TPT
Crystal structures of FNIII domain three and four of the human leucocyte common antigen-related protein, LAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 63.750, 63.750, 236.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 3.200 |
| Rwork | 0.220 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2edx |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.424 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 5225 | 493 |
| <I/σ(I)> | 14.98 | |
| Completeness [%] | 99.3 | |
| Redundancy | 12.2 | |
| CC(1/2) | 0.998 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 0.2 M Sodium fluoride, 0.1 M Bis-tris propane pH 6.5, and 20% PEG3350 |
