6TPI

EnvC bound to the FtsX periplasmic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	67.031, 100.470, 107.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.820 - 2.100
R-factor	0.2177
Rwork	0.215
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4BH5 and 4N8O
RMSD bond length	0.007
RMSD bond angle	1.476
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.823	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.044	0.577
Number of reflections	43252	3494
<I/σ(I)>	15	2.2
Completeness [%]	100.0	100
Redundancy	12	10.8
CC(1/2)	0.999	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	10% PEG 8000, 20 % Ethylene glycol, 0.1 M Tris/bicine pH8.5, ) 0.12M monosaccarides (glucose, mannose, galactose, fucose, xylose, NAG)